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For quantitative PCR (qPCR), total RNA was reverse transcribed into complementary DNA using a Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Scientific, K1682) following the manufacturers instruction

de Groot, and E

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JC virus is an etiologic agent of progressive multifocal leukoencephalopathy (PML), a demyelinating disease

A duration of at least 72 weeks is typical, in part because fibrosis remodels slowly and a shorter study risks missing a real but delayed effect the same lag that likely contributed to semaglutides neutral fibrosis result in its 72-week phase 2 trial