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Description
Transwell migration and invasion assays 1 10 5 HCC cells were seeded in upper chamber suspended in serum-free medium for migration assay, whereas 20% of Matrigel-coated upper chamber was used for invasion assay
Additional file 2: Table S1
The general guideline is to wait at least 24 hours , especially if sedation or pain medicine was given
In addition, western blot analysis was conducted to evaluate the expression levels of renal injury marker protein neutrophil gelatinase-associated lipocalin (NGAL) and pro-inflammatory cytokine interleukin-6 (IL-6) in kidney tissues
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