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Description
There have been numerous environmental agents that have been employed to develop animal models of ASDs (Sever et al., 2022
Common regimens include 50 mg/kg intraperitoneally twice daily for 1228 days in C57BL/6J or ob/ob mice, with indirect calorimetry and body-composition endpoints

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HEK293T, PANC-1, LS174T cells were cultured in high glucose Dulbeccos modified Eagles medium (DMEM)
This change makes the cells more responsive to localized growth signals, enhancing collagen synthesis and structural remodeling