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If the released payload is capable of permeating cell membranes or is transmembrane in nature, it has the potential to trigger a bystander effect: the cleavable connector can release its payload outside the cell within the tumor tissue and can kill adjacent tumor cells with low or negative expression of the target antigen to enhance the efficacy of ADC ( Figure 1 2.3 Advantages and challenges of ADCs ADCs constitute an emerging category of oncological therapeutics that synergistically combine the specificity of monoclonal antibodies with the potent cytotoxic effects of low-molecular-weight agents

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After 3 months of treatment (when the mice reached the early stage of the disease), TRF-treated Q175 mice showed improved motor activity rhythms and sleepwake cycles