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A lot depends on your current B12 levels and the results you're aiming for
Two hundred microliters of the reaction mixture consisting of a final concentration of 1 mM CDNB dissolved in methanol, 5 mM glutathione, and 120 M recombinant GSTs in 100 mM Tris-HCl (pH 7.5) or without recombinant GST for the blank was tested in a 96-well plate
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doi: 10.1016/S0021-9258(17)30124-2
10.1104/pp.001362 98 PanchukI
