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Serum creatinine concentrations were determined using an anzymatic method (sarcosine oxidase-PAP) with a coefficient of variation of 1.1%

Each gene expression experiment was performed at least 3 times and calculations were made from measurements of 3 replicates of each sample

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Calanna S, Christensen M, Holst JJ, Laferrre B, Gluud LL, Vilsbll T, et al