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369 doi: (Published 24 April 2020) Cite this as: BMJ 2020;369:m1319 Muhajir Mohamed, consultant haematologist and associate professor of medicine1 2, Joel Thio, medical intern3, Remy Susan Thomas, medical intern4, Jehan Phillips, general practitioner5 1 Department of Medicine, Launceston General Hospital, Australia 2 University of Tasmania, Launceston Clinical School, Launceston, Australia 3 Department of Medicine, Launceston General Hospital, Australia

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The Bradford assay was used to determine protein concentration of cell extracts, from there samples with the same amount of protein were separated using SDS-PAGE technology and transferred onto a PVDF membrane

The connection to NAD+ biology adds another dimension of interest

While glucose transporter-mediated uptake was experimentally validated in budding yeast, the specific mechanism underlying BBB translocation in vertebrates remains inferred rather than directly demonstrated