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All PCRs were carried out with 15 L of Phusion High-Fidelity PCR Master Mix (New England Biolabs), 0.2 M forward and reverse primers, and approximately 10 ng of template DNA
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Identification of urinary metabolites that correlate with clinical improvements in children with autism treated with sulforaphane from broccoli
Top SNP included rs7251570, rs76112798 and rs11667314, which were most prevalent in donors of Asian or European descent and significantly influenced paraxanthine metabolism ( Online Supplementary Figure S2C, D )
