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It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when theinhibition ratiois 10%~50% (theoptimalinhibition ratio is the range of 25%~45%.) before formal experiment

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Pre-reconstituted and ready to use no mixing required

Salt is helpful in preventing contaminating proteins from binding nonspecifically to the nickel agarose beads and to your his-tagged protein of interest