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Testing is worth doing when: The sore is painless, firm, and has a well-defined raised border (possible chancre) It has lasted longer than two weeks without healing or is growing rather than shrinking You also notice swollen lymph nodes, a rash on the palms or soles, or general flu-like symptoms (possible secondary syphilis) You had oral sex with a new or untested partner and want broader STI screening You want a baseline because you are starting a new sexual relationship For syphilis, a blood antibody test is the at-home and clinical standard

cDNA was amplified using a real-time PCR machine (QuantStudio, Thermo Fisher Scientific) with primers shown in Table S9

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Use Lukewarm Water: Always use lukewarm water to bathe your baby, as hot water can dry out delicate skin