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Description
In both genome-wide and small-scale dropout screens, a positive control (untransduced cells) and a negative control (untransduced cells treated with puromycin) were included for each replicate of each CRISPR screen in all bovine cell lines

M., Carney, S

doi: 10.1016/S0028-3908(98)00162-2 61 WikstrmH

Overall, an activation time of 3 h was set for further experiments, however in special occasions which fast APC production is in favor, reduction of the incubation time to 1 h might result in the same APC production
Heat-shocked (43C for 30 min) utricles were used as positive controls for HSP induction
