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All TMAO concentrations were also made up in Seahorse XFe base media, as were the final optimised concentrations of the stress test compounds: oligomycin (1 M), FCCP (0.25 M), rotenone (1 M), and antimycin A (1 M)

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LDLR / global knockout mice were housed in the same environment as C57BL/6J mice, with free access to water and a high-cholesterol diet (HCD

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