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105 (suppl 2): 417436

Immunoblot assays Tissue samples were crushed on dry ice to obtain homogenous aliquots and then lysed in RIPA buffer (Thermo Scientific #89900) containing Halt protease & phosphatase inhibitor (Thermo Scientific #1861280)

The FDA specifies that household alternatives should be puncture-resistant, have a tight-fitting lid, remain upright during use, and be labeled "Do Not Recycle" or "Used Sharps." Do not use glass containers, thin plastic bottles, or any container that a child could open

MK-677 has multiple human RCTs (including studies by Murphy et al