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Finally, whole cell fractions and beads were either stored at 20 C or denaturised (LDS Sample buffer, 3% -mercaptoethanol, 20 mM DTT, 2 mM biotin, 10 min at 95 C) and resolved by SDS-PAGE

Vitamin C acts as a cofactor for the enzymes prolyl hydroxylase and lysyl hydroxylase, which are required for collagen formation

Thank you and Happy New Year Hi Tony, you may have developed acute brachial neuritis or parsonage turner syndrome see this blog : Im not convinced the cortisone shot was the cause

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