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A QIAGEN midiprep was used to purify these plasmids at a high concentration, so a total of 3 ug DNA could be used per reaction

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As the metabolic pathways related to homocysteine became delineated other important polymorphic variants were described, such as the thermolabile variant of the folate metabolizing enzyme, methylenetetrahydrofolate reductase (MTHFR) (5, 6)

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