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Extracellular flux analysis was conducted using the Seahorse XF Cell Mito Stress Test Kit (Seahorse Bioscience, 103010-100) following the manufacturer's protocol

This plot shows that all levels of depression symptom severity (mild, moderate, severe) shifted away from the WNL centroid but with much overlap between curves for mild and moderate depressive symptoms, and less overlap with the curve for severe depressive symptoms

So by the end of the day, not an hour has gone by that Im not figuring out, OK, am I hanging from something

But in retrospect, it is clear that certain aspects of this design have been flawed since the get-go

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