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Nonetheless, further large-scale, long-term clinical trials are warranted to standardize intermittent fasting protocols, clarify optimal fasting regimens, and evaluate sustained outcomes across different populations

This mechanism may be due to the high enrichment of miR-200a in EVs derived from HESCs, which downregulates Keap1, a negative regulator of Nrf2 expression, thereby playing a crucial role in the angiogenic process mediated by these vesicles ( 3.4 The role of EVs in the development of asthenozoospermia Asthenozoospermia (AS) is one of the common causes of male infertility, characterized by a lower proportion of progressively motile sperm in semen parameters than the reference limit

Gene Set Enrichment Analysis (GSEA) showed enrichment of oxidative phosphorylation, glutathione metabolism, longevity regulating pathways, the NF-B signaling pathway and the TNF signaling pathway (Extended Data Fig