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After incubation with the appropriate antibodies for 30 min, erythrocytes were lysed with lysis buffer (BD FACS Lysing Solution, BD Biosciences, San Jose, USA) for 10 min on a shaker, followed by centrifugation (250 x g, 5 min, 4C, Centrifuge 5427 R, Eppendorf SE, Hamburg, Germany) and washing with HBS

Despite the emergence of resistance, there was little concern because of the continuous production of new drug derivatives to manage the issue

However, under certain circumstances, autophagy can consume too much cytoplasmic material and promote apoptosis or necrosis, ultimately leading to autophagic cell death (Mario et al., 2014)

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