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Alternative reading frames of the INK4a tumor suppressor gene encode two unrelated proteins capable of inducing cell cycle arrest

Naturally occurring First isolated from the brain in 1977
LysoTracker red DND99 (Introgen, Eugene, Oregon, USA), an acidotropic dye and a weak base conjugated to a red fluorophore, was added to the cells on the designated coverglass wells on the day of infection

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