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D., Nguyen H
Kahn, J
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Tissue was lysed in RIPA lysis and extraction buffer (G Biosciences, 786-489) supplemented with 1% Halt protease inhibitor cocktail (Thermo Scientific, 78429) and 1% phosphatase inhibitor cocktail A (Alfa Aesar, J65354.LQ) and Precellys soft tissue homogenizing ceramic beads (Cayman Chemical Company, 10011152) in a Precellys Evolution homogenizer (Bertin Technologies)
Once our cells degrade cysteine to taurine or sulfate, the cysteine is irrecoverable
