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The cells were pelleted by centrifugation and lysed by sonication in 25 mM Tris pH 7.5, 500 mM NaCl, supplemented with 1 mM phenylmethylsulfonyl fluoride and complete (EDTA-free) protease inhibitor tablets (1 tablet per 40 mL of sonication buffer, Roche)
Meier C., Frank C., Grben B., Schack T
NAPQI production estimated via the elimination of glutathione-derived metabolites ( i.e
[DOI] [PubMed] [Google Scholar] 106.Saha PK, Kojima H, Martinez-Botas J, Sunehag AL, Chan L
doi:10.1196/annals.1417.006
