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By in situ hybridization and immunostaining, BPC 157 was found in gastrointestinal mucosa, lung bronchial epithelium, epidermal layer of the skin, and kidney glomeruli [10]
RNA quantification was done by measuring the absorbance at the 260/280 ratio and further it was reverse-transcribed into cDNA and later RT-qPCR was done to amplify and analyse cDNA using specific gene primers and SYBR green (detection dye)
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Sato H, Singer RH
