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The worms were permeabilized and fixed after being resuspended in 400 l of PBS to which an equal volume of 2 MRWB (160 mM KCl, 40 mM NaCl, 14 mM Na 2 EGTA, 1 mM spermidine-HCl, 0.4 mM spermine, 30 mM Na-PIPES pH 7.4, 0.2% -mercaptoethanol) buffer containing 4% paraformaldehyde (PFA) was added

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