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The metabolic effects of insulin cannot be fully compensated by insulin-like growth factor-1 (IGF-1) or by alternative mechanisms [185,186]

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Common problems in GST tag protein purification Non-binding or weak binding of GST tag proteins Possible reasons for inefficient elution of GST tag proteins High level of heteroproteins in purified product Conclusion Generally speaking, purity of GST tag proteins via one-step affinity chromatography will be higher than that of His tag protein via one-step purification

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The role of inflammation in the pathogenesis of osteoarthritis