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Protein concentration of the cell extracts was determined by Bradford assay (Bio-Rad Laboratories, CA, USA)

Downsides of using Vitamin C to treat hyperpigmentation are: Does not effectively inhibit tyrosinase Has difficulty penetrating into the skin Unstable Reacts with other skin care products Has a low pH that can sting skin and affect other products Expensive However, Vitamin C is a great antioxidant and it helps increase skin collagen and prevent aging

doi: 10.1155/2017/1297658 101 VinelliV.BiscottiP.MartiniD.Del BoC.MarinoM.MerooT.et al

Therefore, disease management currently relies primarily on lifestyle interventions and on pharmacological treatment of associated metabolic comorbidities, such as hyperglycemia, dyslipidemia, and cardiovascular risk factors, rather than on drugs directly targeting the full MASLD spectrum [35]