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Description
Free radical biology & medicine 42 , 14701480, doi: 10.1016/j.freeradbiomed.2007.01.044 (2007)

Trichoplusia ni cell pellets were suspended in 30 mM HEPES (pH 7.4), 50 mM NaCl, 5 mM CaCl 2 , 2 mM MgCl 2 supplemented with protease inhibitors (REF118361 45001, Roche) and benzonase (Merk Millipore)
and 21740002 to Z.X.) and by the National Key R&D Program of China (2017YFD0200501 to Z.X.)

Duration: For visible improvement, continue for at least 812 weeks

Cleanse the site with the alcohol prep by starting in the center and working outward