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Contrarily to the other methods listed before, vaccine-based approaches do not only aim to target senescent cells in tissues, but also senescent immune cells, which can be responsible for age-related diseases 60

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The membranes were then washed several times with water and TBS (PBS, 0.05% Tween-20), a 1 :5000 dilution of horseradish-peroxidase conjugated goat anti-rabbit antiserum was added, and the membranes further incubated for 1 h at room temperature

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