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Description
Equal amounts of protein (40 g) were separated by 7.5 12% SDS-PAGE and then transferred electrophoretically onto polyvinylidene difluoride (PVDF) membranes (Millipore, Bedford, MA, United States)
The membranes were sequentially probed with the primary antibodies (1:5000) at 4 C overnight and corresponding horseradish peroxidase-conjugated secondary antibodies (1:10,000, Pierce at room temperature for 1 h)
No se han observado evidencias de efectos adversos hormonales, hepticos o cardiovasculares en los datos disponibles
Since then, numerous investigations have revealed the effects of GIP on multiple organs [14]
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