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Singly-housed rodents may have increased adiposity 101 and, moreover, singly-housed mice in standard caging can engage in coprophagy of their own feces that may impact their cecal microbiome 102

Once treatment with iron is started, it is important to repeat a CBC and iron panel in 2-3 months to assess response to treatment

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Another time to avoid using DIY bac water is when you are working with a valuable or expensive research compound