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Patients from the same cohort returned for a second follow-up appointment after a washout period of 4 weeks, when they were then assigned the alternative intervention for the second phase of the trial (second treatment period)

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Following centrifugation at 12,000 rpm for 3 min, the supernatant was aspirated, and the sample was filtered through a microporous filter membrane (0.22 m pore size) and stored in a sample injection vial for UPLC-MS/MS analysis

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We advise against using any solution that is not completely clear