glutathione as a reducing agent paper on the impact of agents on protein synthesis in PUREfrex® has been published | Others | News | PUREfrex® Glutathione - an overview |

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doi: 10.1093/nar/gkh696

ROS measurement and apoptosis by flow cytometry The intracellular production of ROS was measured using the cell permeable probe DCFDA, which preferentially measures peroxides

Detection of deposited TCC (C5bC9) was done using a biotinylated mouse monoclonal antibody specific for a C9 neoepitope exposed upon C5b binding (Diatec Monoclonals, DIA 011-01) (diluted 1:5000 in PBS/T/S) together with AP-conjugated streptavidin (diluted 1:5000 in PBS/T/S) (Cytiva Life Sciences) and visualized by addition of AP substrate (Sigma-Aldrich)