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The proteins were purified by sequential protein A affinity adsorption on IgG-agarose (Sigma) followed by 3 C protease elution, HiTrap Heparin HP and finally Superdex 200 Increase 10/300 GL (Cytiva) chromatography on an kta purifier controlled by Unicorn (v.7.6.0.1306) software

In addition, we detected strongly increased production of ROS which is similar to findings of other authors

FIGURE 7 A typical workflow of the isoTOP-ABPP approach employs two different (light and heavy) isotopically labeled cleavable biotin-azide tags that are reacted with IA-alkyne probes

once) to the mice causes significant liver damage and necrosis of cells as evidenced by the elevated serum hepatic enzymes alanine aminotransferase (ALT), aminotransferase (AST), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GT) compared with normal group [3]

Specific Organ Risks If a patient exhibits any alarming signs and symptoms after the surgery, promptly seek medical attention