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Ascorbic acid is a cofactor for enzymes involved in the biosynthesis of collagen (essential for tissue maintenance and repair), carnitine, and neurotransmitters

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doi: 10.1038/nature05026

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Pinch your skin and fat together and gently pull it away from underlying muscle, then insert the needle at a 90 angle (perpendicularly or straight up / down) if your tissue is thick enough