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After reaching 70% confluence, fibroblasts were cultured for 24 h in 1% FBS DMEM and then incubated with TGF1 10 ng/ml (Peprotec, Canada), GHK 10 nM (Sigma, Canada) or growth media control for a further 48 h

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For example, many lipolytic proteins such as PLIN1 (13, 130), PNPLA2 (131, 132) and G0S2 (133) are stabilized by condition that promote LD biogenesis, such as oleic acid supplementation, where association with the phospholipid monolayer is thought to mask motifs that signal degradation