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Where preclinical data is the primary evidence base, that is stated explicitly

The effect of peroxisome proliferator-activated receptor- ligands on in vitro and in vivo models of COPD

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First, AX-protein conjugates were analysed by SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis) and Western blot using an anti-AX monoclonal antibody and, second, the content of low molecular weight compounds was studied by spectroscopic techniques and liquid chromatography

Supplementary Material Abbreviations Used 1,25(OH) 2 VD 1alpha,25-dihydroxyvitamin D3 25(OH)VD 25-hydroxyvitamin D ANOVA analysis of variance BMI body mass index BW body weight cDNA complementary DNA CT threshold cycle CYP cytochrome P450 enzymes DMEM Dulbecco's modified Eagle's medium EDTA ethylenediaminetetraacetic acid ELISA enzyme-linked immunosorbent assay GAPDH glyceraldehyde-3-phosphate dehydrogenase GCL glutamate-cysteine ligase GCLC glutamate-cysteine ligase catalytic subunit GCLM glutamate-cysteine ligase regulatory subunit GLUT-4 glucose transporter type 4 GSH glutathione GM growth medium HFD high-fat diet HOMA homeostatic model assessment HPLC high performance liquid chromatography IR insulin resistance KD knockdown LC L-cysteine MDA malondialdehyde NRF2 nuclear factor erythroid-2-related factor OO olive oil PCR polymerase chain reaction PGC-1 peroxisome proliferator-activated receptor gamma coactivator 1-alpha PTH parathyroid hormone RXR retinoic X receptor TNF- tumor necrosis factor alpha VD vitamin D VDBP vitamin D binding protein VDR vitamin D receptor Footnotes * Acknowledgments The authors are supported by grants from NIH (R01 AT007442) and the Malcolm Feist Endowed Chair in Diabetes