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The molecular basis of G protein-coupled receptor activation

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After 24 h, cells were treated with C5a (diluted in medium) (10 nM, R&D, USA) or PAC (diluted in medium) (10 nM, Sigma, USA) for 24 h, then the media was replaced with culture media (5 ml) for 24 h and finally collected for proteome profiler array

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