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For RNA extraction, tissues were thawed on ice in 500 l of sterile, calcium- and magnesium-free phosphate-buffered saline (PBS

Sakarya S, Rifat S, Zhou J, Bannerman DD, Stamatos NM, Cross AS, Goldblum SE (2004) Mobilization of neutrophil sialidase activity desialylates the pulmonary vascular endothelial surface and increases resting neutrophil adhesion to and migration across the endothelium

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Drug Metab Dispos 35:12621268 Wu H, Hait WN, Yang JM (2003) Small interfering RNA-induced suppression of MDR1 (P-glycoprotein) restores sensitivity to multidrug-resistant cancer cells

Inhibition of Bcl-2 with ABT-263 reversed these effects, resulting in increases of 23.2%, 62.94%, 42.09%, 17.64%, and 36.54%, respectively, compared with HSYA alone (Figures 5GK)