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The total RNA concentration was determined spectrophotometrically in RNAase-free water, and 1 g aliquots of total RNA were reverse transcribed into cDNA using ProtoScript First Strand cDNA Synthesis Kit (NEB)
Research published in Cell Metabolism demonstrated that MOTS-c administration in mice improved insulin sensitivity by 28% and increased running capacity by nearly 50%, effects attributed to enhanced mitochondrial function and glucose regulation
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As for your diet, you can expect significant changes to your appetite and food preferences, especially if youre also managing side effects
