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For all practical research purposes, you can assume the final volume is equal to the volume of bacteriostatic water you added

10.1007/BF01504715 10.1007/BF01504715 [DOI] [Google Scholar] Hadjigogos K (2003) The role of free radicals in the pathogenesis of rheumatoid arthritis

For example, riboflavin is usually present as FAD in a prosthetic group that is complexed to the enzyme and would have to be dissociated during digestion
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doi: 10.1016/j.tox.2007.03.007
