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Description

All in all, the MeOH/MTBE-based LLE is more environmentally friendly (no halogenated solvents) and the collection of the upper phase containing the lipids is easier in comparison to the extraction with CHCl 3 where the lipids are in the lower phase

Statistical analysis for metabolomics was performed with Metaboseek software (v0.9.9.0), Microsoft Excel (v2302 Build 16.0.16130.20332), and with GraphPad Prism (v9.5.0.730)

Lee arrested all the Tories he could find, deprived them of their arms, and imposed upon them the severest restrictions

Sigma-Aldrich) at 10 g/ml (normal cholesterol) or 40 g/ml (high cholesterol) and seeded with Escherichia coli OP50 at 20C as described previously (88)