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The effect is more prominent in case of the trivalent lanthanides which can additionally bind to the central NAC region and accelerate fibril formation ( Native nano-electrospray ionisation and ion mobility-mass spectrometry (nESI(-IM)-MS) can differentiate conformations of the protein based on their charged states, relying on the principle that denatured proteins are more extended and therefore are comparatively more protonated than native compact states with a lower Solvent Accessible Surface Area

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