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Primary antibodies used were: mouse monoclonal (IgG2A) anti-PSD-95 clone K28/43 (1:200, Neuromab, UC Davis, Davis, CA), mouse monoclonal IgG1 anti-PSD-95 clone 7E3-1B8 (1:250, Thermo Fisher Scientific, Waltham, MA, cat#: MA1-046) anti-guinea pig polyclonal anti-Bassoon (1:300, Synaptic Systems, Gottingen, Germany, cat# 141 004), rabbit polyclonal anti-Bassoon (1:300, Synaptic Systems, cat #: 141 003), chicken anti-GFP (1:2000, Abcam, Cambridge, MA, cat# ab13970), rabbit monoclonal anti-GluN1 (1:500, AB9864, Millipore Sigma), mouse monoclonal (IgG2A) anti-GluN2A clone N327/95 (1:250, Neuromab), mouse monoclonal (IgG2B) anti-GluN2B clone N59/36 (1:250, Neuromab), mouse monoclonal (IgG1) anti-GluA1 clone N355/11 (1:250, Neuromab), rabbit polyclonal anti-GluA2(1:500, Synaptic Systems, cat#: 182 103), mouse monoclonal (IgG2A) anti-Synaptotagmin 1 (1:500, Synaptic Systems, cat#: 105 011)

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At low concentrations, NO reactions with organic molecules and oxygen species would translate in NO-induced cell signaling, stimulating the antioxidant stress responses while high NO concentrations would boost nitrogen oxide reactions inflicting severe cell damage

Frequency-wise, begin with once-daily application, preferably at night, and after 1-2 weeks with no irritation, you may increase to twice daily if directed by your dermatologist

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