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In Vitro Pull-Down Binding Assays To determine whether GSTP binds directly to HMGB1, recombinant polyhistidine-tagged GSTP (WT), GSTP (S184A), GSTP (S184D), and HMGB1 proteins were expressed in Escherichia coli BL21 (DE3) and purified through Ni-NTA spin column (Qiagen)
doi: 10.1016/S0764-4469(01)01350-6
Type I collagen is the main component of the ECM
Several of these genes, such as GPX6 (At4g11600), are induced by oxidative stress (eg, Mhamdi et al., 2010a)
