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How Antibiotics Disrupt Gut Microbiota Elimination of Beneficial Bacteria : Antibiotics do not discriminate between harmful pathogens and beneficial bacteria, leading to a decrease in microbial diversity

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10.1152/jappl.1987.63.1.152 [DOI] [PubMed] [Google Scholar] 113.Roum JH, Buhl R, McElvaney NG, Borok Z, Crystal RG

Cell pellets were resuspended in reducing lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 10 mM DTT, 0.01 mg ml 1 DNase I) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator

Liver function in patients with long-term coronavirus disease 2019 of up to 20 months: a cross-sectional study