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Description
The reaction was quenched by transferring 50 L of the sample to 200 L of internal standard solution, followed by homogenization (Eppendorf Mixmate, 1200 rpm, 5 min) and centrifugation (4000 rpm, 15 min, 4C)

May 1995;49(3):191-9

doi: 10.4103/1658-354X.115316

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In childhood-onset patients, the network showed stronger connections between nodes representing disease duration, chronic hyperglycemia, and cognitive functions, suggesting that these factors may contribute to more pronounced cognitive impairments