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A comprehensive review of recent clinical data highlights that while folate deficiency is common among individuals with ASD, maternal folic acid supplementation does not correlate with higher risk of the disorder, indeed folate therapy has been associated with improved neurodevelopmental outcomes (Vasconcelos et al., 2025)

doi: 10.1038/nrd4359 207

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PCR cycling conditions were as follows: 95 C for 3 minutes, followed by 30 cycles of 94 C for 30 seconds, 55 C for 45 seconds, 72 C for 1 minute, with a final extension at 72 C for 7 minutes and holding at 4 C