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IHC was performed by thawing the slides (all steps were performed at RT unless otherwise indicated) in a humidifying tray for 5 min, permeabilized for 15 min with 1 PBS + 0.1% Tween (PBST), and blocked in blocking buffer (PBST + 1% normal donkey serum (NDS)) for 30 min

At the same time, we purified tdTomato-negative striatal cells (which include any other cell type in the striatal tissue) from both controls and mutants (here called iSPN wt and iSPN mut, respectively)

doi: 10.3389/fvets.2021.661380 36 ZhouYLanRXuYZhouYLinXMiaoJ

The lesions are typically associated with localized pain, erythema, or nonpurulent drainage

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