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Treatments for facial synkinesis include facial training mainly based on facial biofeedback retraining, chemodenervation with botulinum toxin, selective neurectomy and/or myectomy, and any combination treatment of these options [16]

A 35 Ultra Semi diamond knife (Diatome) mounted on a UC7 ultramicrotome (Leica) was used to cut alternating series of sections onto formvar-coated copper mesh grids at two different thicknesses: (i) 60 nm-thick sections were visually inspected to assess ultrastructural preservation and synapse density under an 80kV LEO 912 transmission electron microscope (Zeiss), and (ii) 250 nm-thick sections were coated on both surfaces with Protein A-conjugated 10 nm gold fiducial particles (Cell Microscopy Core Products, University Medical Center Utrecht, The Netherlands) in preparation for transmission electron tomography

PubMed Greenwood-Van Meerveld, B., Tyler, K., Mohammadi, E., & Pietra, C

Ethical Considerations: Always prioritize the welfare of your research subjects

2018;24:637652.e638