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If Im cleaving off the affinity tag, then Ill usually do that after the affinity purification and re-run the affinity column again to deplete the cleaved tag and any un-cleaved fusion protein

that you should be careful about when importing individually

doi: 10.1097/01.NAJ.0000731640.35662.2c

Upon its accumulation and phosphorylation, HIF-1 dimerizes with HIF- (constitutively expressed nuclear proteins), enters the nucleus, and promotes the transcription of hypoxia response elements (HRE)-containing target genes involved in energy metabolism, erythropoiesis, vascular remodelling, and other processes (Fig

ESGCT Abstract Author Index
