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50 L of samples or standard were incubated with 50 L of Biotin-labeled Antibody at 37C for 45 min

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Due to its high incidence and disability rates, DKD poses a serious threat to human health and represents a significant public health burden

Moreover, the abundance of GSH (110 mmol L 1 ) might favour the spontaneous reaction between GSH and FA, which, if not limited, could alter the GSH:GSSG ratio leading to oxidative stress